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Extra resources for Apocalisse gioiosa
H. NUGTEREN and H. VONKEMAN Enzymatic conversion of all-cis-polyunsaturated fatty acids into prostaglandins. Nature. 203: 839-841. 1964. 14Carbon labelled homo--y-linolenic acid was incubated with homogenates of sheep seminal vesicles. The reaction products contained radiolabelled PGE 1 . The enzyme system was also able to convert other straight chain, all-cis-polyunsaturated (18 and 19 carbon) fatty acids into the corresponding nor-prostaglandins. The nor-prostaglandins had very little biological activity.
Saturation of the 11 13 double bond and oxidation of the secondary alcohol group at C-15. In swine lung only the latter transformation occurs. To study this reaction more closely, a purified enzyme was prepared from swine lung. It could be shown that the enzyme was specific for the hydroxyl group at C-15 in the prostaglandins and could thus be named 15-hydroxyprostaglandin dehydrogenase. NAD+ but not NADP+ served as co-factor. The possible use of this enzyme in a sensitive and specific assay for prostaglandins is being investigated.
Authors) 0346 0165 RYHAGE, R. and B. SAMUELSSON The origin of oxygen incorporated during the biosynthesis of prostagland in E I Biochemical and Biophysical Research Communications. 19: 279·282. 1965. The origin of the oxygen incorporated during the formation of PGE I from dihomo-),-linolenic acid was studied by incubating sheep seminal vesicle homogenates with arachidonic acid in an 18 0 environment. The PGE I produced was analyzed by mass spectrometry in conjunction with gas chromatography. The results clearly show that the oxygens of the 2 hydroxyl groups come from oxygen gas, but this study does not allow conclusions to be drawn about the origin of the keto oxygen.