By Kouji Ikura, Masaya Nagao, Seiji Masuda, Ryuzo Sasaki
Animal phone know-how is a becoming self-discipline of mobilephone biology which goals not just to appreciate buildings, services and behaviors of differentiated animal cells but in addition to examine their talents for use in business and scientific reasons. The objective of animal mobile expertise comprises accomplishments of clonal growth of differentiated cells with necessary skill, optimization in their tradition stipulations, modulation in their skill to supply medically and pharmaceutically, very important proteins, and the applying of animal cells to gene remedy and synthetic organs. This quantity provides the readers a whole overview of current state-of-the-art in Japan. The lawsuits should be necessary for mobilephone biologists, biochemists, molecular biologists, immunologists, biochemical engineers and different disciplines on the topic of animal mobile tradition, operating in both educational environments or in biotechnology and pharmaceutical industries.
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Extra info for Animal Cell Technology: Challenges for the 21st Century, 1st Edition
In Cell volume increased with increase osmotic pressure in both suspension and adhesion cultures (Fig. 2) but no morphological change was noted in the suspension culture, in contrast, cell height 33 34 decreased and cell adhesion area markedly increased with increase in osmotic pressure in the adhesion culture (Figs. 2, 3). This morphological change in adhesion cultures could be one explanation for the higher sensitivity of adherent cells to increase of osmotic pressure than suspended cells. Osmotic Fig.
This concept is realised in the Membrane Dialysis Reactor (Bioengineering, CH) shown in figure 2. Figure 2: Membrane Dialysis Reactor 5. 5-fold in viable cell concentration Xv (Fig. 3) and 3-fold in antibody concentration CMAb (Fig. 4b) compared to the conventional batch. The strategy was not able to avoid the accumulation of metabolites such as ammonia after 80 h of cultivation and culture growth was inhibited. 26 ON-LINE FEEDING STRATEGY FOR HYBRIDOMA CULTURES The dialysis fed-batch combines the on-line feeding strategy with the concept of ‘nutrient-split’ feeding to supply essential substrate directly to the culture and reduce the effect of inhibiting metabolites by dialysis.
Journal of Chemical Technology andBiotechnology 32,324-329. , Meier, J. I. (1985) Mechanistic analysis ofthe inoculum requirement for the cultivation of mammalian cells on microcarriers. Biotechnology and Bioengineering 27, 585-595. G. and Noé, W. (1997) Investigation on oxygen limitations adherent cells growing on macroporous microcarriers. Cytotechnology 24,121-134. This page intentionally left blank. W. Y. W. K. S. H. G. H. Y. PARK1 1Biological Production Unit; Central Research Center, Korea Green Cross Corpration, 22 7 Kaigal-Xi, Kiheung-Eup, Yongin-Si, Kyunggi-Do, 449-900, Korea 2DepartmentofAgricultural Chemistry, Korea University, Seoul 136-701, Korea We cultivated the hybridoma producing vWF antibody in a 10L bioreactor in order to investigate the characteristics of two different cultures, intermittent and continuous bleeding.